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itaq universal sybr green supermix  (Bio-Rad)


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    Bio-Rad itaq universal sybr green supermix
    Itaq Universal Sybr Green Supermix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1631 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/itaq+sybr+green+supermix/ITaq+Universal+SYBR+Green+Supermix/pm42893963-66-4-9
    Average 99 stars, based on 1631 article reviews
    itaq universal sybr green supermix - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Molecular biomarkers and targets for Fuchs' endothelial corneal dystrophy and glaucoma
    Article Snippet: The amplification products were separated by 1.5% agarose gel electrophoresis. qPCR experiments were performed on a 7500 real-time PCR system (Applied Biosystems) using iTaq SYBR Green Supermix (Bio-Rad). .. The amplification products were separated by 1.5% agarose gel electrophoresis. qPCR experiments were performed on a 7500 real-time PCR system (Applied Biosystems) using iTaq SYBR Green Supermix (Bio-Rad). ..

    Article Title: Cross-species transcriptomic analysis unveils conserved host responses in Solanum tuberosum and Solanum lycopersicum against Candidatus Liberibacter solanacearum
    Article Snippet: .. RT-qPCR was performed using a cocktail of iTaq SYBR Green Supermix (BioRad Laboratories, Hercules, CA), cDNA template, and a primer set, and was amplified in three biological replicates per sample. ..

    Article Title: Differential spleen immune signatures and germinal center responses during acute infection with Orientia tsutsugamushi Karp versus Gilliam strains
    Article Snippet: The RNEasy Mini kit (Qiagen) was used for total RNA extraction i Kit (Qiagen). .. The iScript Reverse Transcription kit (Bio-Rad) was used for cDNA synthesis and amplification in a 10 μL-reaction mixture containing 5 μL of iTaq SYBR Green Supermix (Bio-Rad) and 0.5 μM each of gene-specific forward and reverse primers. qRT-PCR assays were performed on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad), and PCR assays were denatured for 30s at 95 °C, followed by 40 cycles of 15s at 95 °C, and 60s at 60 °C. ..

    Agarose Gel Electrophoresis:

    Article Title: Molecular biomarkers and targets for Fuchs' endothelial corneal dystrophy and glaucoma
    Article Snippet: The amplification products were separated by 1.5% agarose gel electrophoresis. qPCR experiments were performed on a 7500 real-time PCR system (Applied Biosystems) using iTaq SYBR Green Supermix (Bio-Rad). .. The amplification products were separated by 1.5% agarose gel electrophoresis. qPCR experiments were performed on a 7500 real-time PCR system (Applied Biosystems) using iTaq SYBR Green Supermix (Bio-Rad). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Molecular biomarkers and targets for Fuchs' endothelial corneal dystrophy and glaucoma
    Article Snippet: The amplification products were separated by 1.5% agarose gel electrophoresis. qPCR experiments were performed on a 7500 real-time PCR system (Applied Biosystems) using iTaq SYBR Green Supermix (Bio-Rad). .. The amplification products were separated by 1.5% agarose gel electrophoresis. qPCR experiments were performed on a 7500 real-time PCR system (Applied Biosystems) using iTaq SYBR Green Supermix (Bio-Rad). ..

    Article Title: Sex differences in hepatitis A virus infection in mice.
    Article Snippet: A NanoDrop (Thermo Scientific, Wilmington, DE) was used to measure RNA concentration. .. HAV genomic RNA was quantified by a 2-step reverse-transcription quantitative PCR procedure using Super-Script-III First-Strand Synthesis Supermix (Invitrogen), iTaq SYBR Green Supermix (Bio-Rad), and a CFX96 RealTime PCR Detection System (Bio-Rad). ..

    Article Title: Differential spleen immune signatures and germinal center responses during acute infection with Orientia tsutsugamushi Karp versus Gilliam strains
    Article Snippet: The RNEasy Mini kit (Qiagen) was used for total RNA extraction i Kit (Qiagen). .. The iScript Reverse Transcription kit (Bio-Rad) was used for cDNA synthesis and amplification in a 10 μL-reaction mixture containing 5 μL of iTaq SYBR Green Supermix (Bio-Rad) and 0.5 μM each of gene-specific forward and reverse primers. qRT-PCR assays were performed on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad), and PCR assays were denatured for 30s at 95 °C, followed by 40 cycles of 15s at 95 °C, and 60s at 60 °C. ..

    Article Title: Selective Activation of Macrophage Innate Signaling Pathways and Inflammatory Responses to Orientia tsutsugamushi Karp and Gilliam Strains
    Article Snippet: .. To determine host gene expression, total RNA of MΦ was extracted via RNeasy mini kit (Qiagen), and cDNA was synthesized utilizing an iScript cDNA kit. qRT-PCR assays were performed using iTaq SYBR Green Supermix (Bio-Rad) on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). ..

    SYBR Green Assay:

    Article Title: Molecular biomarkers and targets for Fuchs' endothelial corneal dystrophy and glaucoma
    Article Snippet: The amplification products were separated by 1.5% agarose gel electrophoresis. qPCR experiments were performed on a 7500 real-time PCR system (Applied Biosystems) using iTaq SYBR Green Supermix (Bio-Rad). .. The amplification products were separated by 1.5% agarose gel electrophoresis. qPCR experiments were performed on a 7500 real-time PCR system (Applied Biosystems) using iTaq SYBR Green Supermix (Bio-Rad). ..

    Article Title: TREM2 promotes adipogenesis of PDGFR-α + adipose stem cells but is dispensable for adipose remodeling and metabolic health during diet-induced obesity
    Article Snippet: .. Reverse transcription into cDNA was conducted using the iScript cDNA Synthesis Kit according to the manufacturer’s instructions (#170-8891, Bio-Rad). qRT-PCR was performed using the iTaq SYBR Green Supermix with ROX according to the supplier (# 72-5853, Bio-Rad). .. PCR was performed with the StepOnePlusTM Real-Time PCR System (Applied Biosystems).

    Article Title: Coordinated transcriptional reprogramming mediates drought tolerance in potato, revealed by temporally integrated network analysis
    Article Snippet: .. The RT-qPCR reactions contained a cocktail of iTaq SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA), cDNA template, and a primer set. ..

    Article Title: Cross-species transcriptomic analysis unveils conserved host responses in Solanum tuberosum and Solanum lycopersicum against Candidatus Liberibacter solanacearum
    Article Snippet: .. RT-qPCR was performed using a cocktail of iTaq SYBR Green Supermix (BioRad Laboratories, Hercules, CA), cDNA template, and a primer set, and was amplified in three biological replicates per sample. ..

    Article Title: Sex differences in hepatitis A virus infection in mice.
    Article Snippet: A NanoDrop (Thermo Scientific, Wilmington, DE) was used to measure RNA concentration. .. HAV genomic RNA was quantified by a 2-step reverse-transcription quantitative PCR procedure using Super-Script-III First-Strand Synthesis Supermix (Invitrogen), iTaq SYBR Green Supermix (Bio-Rad), and a CFX96 RealTime PCR Detection System (Bio-Rad). ..

    Article Title: Differential spleen immune signatures and germinal center responses during acute infection with Orientia tsutsugamushi Karp versus Gilliam strains
    Article Snippet: The RNEasy Mini kit (Qiagen) was used for total RNA extraction i Kit (Qiagen). .. The iScript Reverse Transcription kit (Bio-Rad) was used for cDNA synthesis and amplification in a 10 μL-reaction mixture containing 5 μL of iTaq SYBR Green Supermix (Bio-Rad) and 0.5 μM each of gene-specific forward and reverse primers. qRT-PCR assays were performed on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad), and PCR assays were denatured for 30s at 95 °C, followed by 40 cycles of 15s at 95 °C, and 60s at 60 °C. ..

    Article Title: Selective Activation of Macrophage Innate Signaling Pathways and Inflammatory Responses to Orientia tsutsugamushi Karp and Gilliam Strains
    Article Snippet: .. To determine host gene expression, total RNA of MΦ was extracted via RNeasy mini kit (Qiagen), and cDNA was synthesized utilizing an iScript cDNA kit. qRT-PCR assays were performed using iTaq SYBR Green Supermix (Bio-Rad) on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). ..

    Article Title: Inducible nitric oxide synthase (iNOS) regulates skin eschar lesions, bacterial persistence, and inflammatory resolution in mouse models of scrub typhus
    Article Snippet: Total RNA was extracted from mouse tissues using the RNeasy Mini Kit (Qiagen). .. RNA concentration and purity were assessed using a BioTek microplate reader; cDNA was synthesized from 1 μg of total RNA using the iScript Reverse Transcription Kit (Bio-Rad). qRT-PCR reactions (10 μL in total volume) contained 5 μL of iTaq SYBR Green Supermix (Bio-Rad), 1 μL of forward and reverse primer mix (final concentration 0.5 μM each), and 4 μL of diluted cDNA. .. Amplification was carried out on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) with the following cycling conditions: initial denaturation at 95°C for 30s, followed by 40 cycles of 95°C for 15s and 60°C for 60s.

    Reverse Transcription:

    Article Title: TREM2 promotes adipogenesis of PDGFR-α + adipose stem cells but is dispensable for adipose remodeling and metabolic health during diet-induced obesity
    Article Snippet: .. Reverse transcription into cDNA was conducted using the iScript cDNA Synthesis Kit according to the manufacturer’s instructions (#170-8891, Bio-Rad). qRT-PCR was performed using the iTaq SYBR Green Supermix with ROX according to the supplier (# 72-5853, Bio-Rad). .. PCR was performed with the StepOnePlusTM Real-Time PCR System (Applied Biosystems).

    Article Title: Sex differences in hepatitis A virus infection in mice.
    Article Snippet: A NanoDrop (Thermo Scientific, Wilmington, DE) was used to measure RNA concentration. .. HAV genomic RNA was quantified by a 2-step reverse-transcription quantitative PCR procedure using Super-Script-III First-Strand Synthesis Supermix (Invitrogen), iTaq SYBR Green Supermix (Bio-Rad), and a CFX96 RealTime PCR Detection System (Bio-Rad). ..

    Article Title: Differential spleen immune signatures and germinal center responses during acute infection with Orientia tsutsugamushi Karp versus Gilliam strains
    Article Snippet: The RNEasy Mini kit (Qiagen) was used for total RNA extraction i Kit (Qiagen). .. The iScript Reverse Transcription kit (Bio-Rad) was used for cDNA synthesis and amplification in a 10 μL-reaction mixture containing 5 μL of iTaq SYBR Green Supermix (Bio-Rad) and 0.5 μM each of gene-specific forward and reverse primers. qRT-PCR assays were performed on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad), and PCR assays were denatured for 30s at 95 °C, followed by 40 cycles of 15s at 95 °C, and 60s at 60 °C. ..

    Article Title: Inducible nitric oxide synthase (iNOS) regulates skin eschar lesions, bacterial persistence, and inflammatory resolution in mouse models of scrub typhus
    Article Snippet: Total RNA was extracted from mouse tissues using the RNeasy Mini Kit (Qiagen). .. RNA concentration and purity were assessed using a BioTek microplate reader; cDNA was synthesized from 1 μg of total RNA using the iScript Reverse Transcription Kit (Bio-Rad). qRT-PCR reactions (10 μL in total volume) contained 5 μL of iTaq SYBR Green Supermix (Bio-Rad), 1 μL of forward and reverse primer mix (final concentration 0.5 μM each), and 4 μL of diluted cDNA. .. Amplification was carried out on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) with the following cycling conditions: initial denaturation at 95°C for 30s, followed by 40 cycles of 95°C for 15s and 60°C for 60s.

    cDNA Synthesis:

    Article Title: TREM2 promotes adipogenesis of PDGFR-α + adipose stem cells but is dispensable for adipose remodeling and metabolic health during diet-induced obesity
    Article Snippet: .. Reverse transcription into cDNA was conducted using the iScript cDNA Synthesis Kit according to the manufacturer’s instructions (#170-8891, Bio-Rad). qRT-PCR was performed using the iTaq SYBR Green Supermix with ROX according to the supplier (# 72-5853, Bio-Rad). .. PCR was performed with the StepOnePlusTM Real-Time PCR System (Applied Biosystems).

    Article Title: Differential spleen immune signatures and germinal center responses during acute infection with Orientia tsutsugamushi Karp versus Gilliam strains
    Article Snippet: The RNEasy Mini kit (Qiagen) was used for total RNA extraction i Kit (Qiagen). .. The iScript Reverse Transcription kit (Bio-Rad) was used for cDNA synthesis and amplification in a 10 μL-reaction mixture containing 5 μL of iTaq SYBR Green Supermix (Bio-Rad) and 0.5 μM each of gene-specific forward and reverse primers. qRT-PCR assays were performed on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad), and PCR assays were denatured for 30s at 95 °C, followed by 40 cycles of 15s at 95 °C, and 60s at 60 °C. ..

    Quantitative RT-PCR:

    Article Title: TREM2 promotes adipogenesis of PDGFR-α + adipose stem cells but is dispensable for adipose remodeling and metabolic health during diet-induced obesity
    Article Snippet: .. Reverse transcription into cDNA was conducted using the iScript cDNA Synthesis Kit according to the manufacturer’s instructions (#170-8891, Bio-Rad). qRT-PCR was performed using the iTaq SYBR Green Supermix with ROX according to the supplier (# 72-5853, Bio-Rad). .. PCR was performed with the StepOnePlusTM Real-Time PCR System (Applied Biosystems).

    Article Title: Coordinated transcriptional reprogramming mediates drought tolerance in potato, revealed by temporally integrated network analysis
    Article Snippet: .. The RT-qPCR reactions contained a cocktail of iTaq SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA), cDNA template, and a primer set. ..

    Article Title: Cross-species transcriptomic analysis unveils conserved host responses in Solanum tuberosum and Solanum lycopersicum against Candidatus Liberibacter solanacearum
    Article Snippet: .. RT-qPCR was performed using a cocktail of iTaq SYBR Green Supermix (BioRad Laboratories, Hercules, CA), cDNA template, and a primer set, and was amplified in three biological replicates per sample. ..

    Article Title: Differential spleen immune signatures and germinal center responses during acute infection with Orientia tsutsugamushi Karp versus Gilliam strains
    Article Snippet: The RNEasy Mini kit (Qiagen) was used for total RNA extraction i Kit (Qiagen). .. The iScript Reverse Transcription kit (Bio-Rad) was used for cDNA synthesis and amplification in a 10 μL-reaction mixture containing 5 μL of iTaq SYBR Green Supermix (Bio-Rad) and 0.5 μM each of gene-specific forward and reverse primers. qRT-PCR assays were performed on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad), and PCR assays were denatured for 30s at 95 °C, followed by 40 cycles of 15s at 95 °C, and 60s at 60 °C. ..

    Article Title: Selective Activation of Macrophage Innate Signaling Pathways and Inflammatory Responses to Orientia tsutsugamushi Karp and Gilliam Strains
    Article Snippet: .. To determine host gene expression, total RNA of MΦ was extracted via RNeasy mini kit (Qiagen), and cDNA was synthesized utilizing an iScript cDNA kit. qRT-PCR assays were performed using iTaq SYBR Green Supermix (Bio-Rad) on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). ..

    Article Title: Inducible nitric oxide synthase (iNOS) regulates skin eschar lesions, bacterial persistence, and inflammatory resolution in mouse models of scrub typhus
    Article Snippet: Total RNA was extracted from mouse tissues using the RNeasy Mini Kit (Qiagen). .. RNA concentration and purity were assessed using a BioTek microplate reader; cDNA was synthesized from 1 μg of total RNA using the iScript Reverse Transcription Kit (Bio-Rad). qRT-PCR reactions (10 μL in total volume) contained 5 μL of iTaq SYBR Green Supermix (Bio-Rad), 1 μL of forward and reverse primer mix (final concentration 0.5 μM each), and 4 μL of diluted cDNA. .. Amplification was carried out on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) with the following cycling conditions: initial denaturation at 95°C for 30s, followed by 40 cycles of 95°C for 15s and 60°C for 60s.

    Polymerase Chain Reaction:

    Article Title: Sex differences in hepatitis A virus infection in mice.
    Article Snippet: A NanoDrop (Thermo Scientific, Wilmington, DE) was used to measure RNA concentration. .. HAV genomic RNA was quantified by a 2-step reverse-transcription quantitative PCR procedure using Super-Script-III First-Strand Synthesis Supermix (Invitrogen), iTaq SYBR Green Supermix (Bio-Rad), and a CFX96 RealTime PCR Detection System (Bio-Rad). ..

    Article Title: Differential spleen immune signatures and germinal center responses during acute infection with Orientia tsutsugamushi Karp versus Gilliam strains
    Article Snippet: The RNEasy Mini kit (Qiagen) was used for total RNA extraction i Kit (Qiagen). .. The iScript Reverse Transcription kit (Bio-Rad) was used for cDNA synthesis and amplification in a 10 μL-reaction mixture containing 5 μL of iTaq SYBR Green Supermix (Bio-Rad) and 0.5 μM each of gene-specific forward and reverse primers. qRT-PCR assays were performed on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad), and PCR assays were denatured for 30s at 95 °C, followed by 40 cycles of 15s at 95 °C, and 60s at 60 °C. ..

    Gene Expression:

    Article Title: Selective Activation of Macrophage Innate Signaling Pathways and Inflammatory Responses to Orientia tsutsugamushi Karp and Gilliam Strains
    Article Snippet: .. To determine host gene expression, total RNA of MΦ was extracted via RNeasy mini kit (Qiagen), and cDNA was synthesized utilizing an iScript cDNA kit. qRT-PCR assays were performed using iTaq SYBR Green Supermix (Bio-Rad) on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). ..

    Synthesized:

    Article Title: Selective Activation of Macrophage Innate Signaling Pathways and Inflammatory Responses to Orientia tsutsugamushi Karp and Gilliam Strains
    Article Snippet: .. To determine host gene expression, total RNA of MΦ was extracted via RNeasy mini kit (Qiagen), and cDNA was synthesized utilizing an iScript cDNA kit. qRT-PCR assays were performed using iTaq SYBR Green Supermix (Bio-Rad) on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). ..

    Article Title: Inducible nitric oxide synthase (iNOS) regulates skin eschar lesions, bacterial persistence, and inflammatory resolution in mouse models of scrub typhus
    Article Snippet: Total RNA was extracted from mouse tissues using the RNeasy Mini Kit (Qiagen). .. RNA concentration and purity were assessed using a BioTek microplate reader; cDNA was synthesized from 1 μg of total RNA using the iScript Reverse Transcription Kit (Bio-Rad). qRT-PCR reactions (10 μL in total volume) contained 5 μL of iTaq SYBR Green Supermix (Bio-Rad), 1 μL of forward and reverse primer mix (final concentration 0.5 μM each), and 4 μL of diluted cDNA. .. Amplification was carried out on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) with the following cycling conditions: initial denaturation at 95°C for 30s, followed by 40 cycles of 95°C for 15s and 60°C for 60s.

    Concentration Assay:

    Article Title: Inducible nitric oxide synthase (iNOS) regulates skin eschar lesions, bacterial persistence, and inflammatory resolution in mouse models of scrub typhus
    Article Snippet: Total RNA was extracted from mouse tissues using the RNeasy Mini Kit (Qiagen). .. RNA concentration and purity were assessed using a BioTek microplate reader; cDNA was synthesized from 1 μg of total RNA using the iScript Reverse Transcription Kit (Bio-Rad). qRT-PCR reactions (10 μL in total volume) contained 5 μL of iTaq SYBR Green Supermix (Bio-Rad), 1 μL of forward and reverse primer mix (final concentration 0.5 μM each), and 4 μL of diluted cDNA. .. Amplification was carried out on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad) with the following cycling conditions: initial denaturation at 95°C for 30s, followed by 40 cycles of 95°C for 15s and 60°C for 60s.



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